Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/07/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | North Penn-Mansfield JSHS |
| Observations | Location – On a rock, part of the riverbed Notes – None |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius neoniger |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | All controls returned as expected, test lane has one clearly defined line in parallel with the negative arthropod control. However, there is an extremely faint second line in the test lane that could indicate a positive result. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lasius neoniger was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid