Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 02/12/2025 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Berkshire Community College |
| Observations | This Blaptica Dubia roach was purchased from a local Petco store, where it was part of a captive colony.
|
| Putative identification | Arthropoda Insecta Blattodea Blaberidae Blaptica Blaptica dubia |
Methods |
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| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | For the DNA extraction we used the insect adaptation of the protocol On March 4th 2025 we did a single PCR with Arthropod primers which used: Taq polymerase used: New England Bio Labs One Taq Annealing temperatures for PCR reaction: 49 C On March 25th 2025 we did a duplex PCR with Arthropod and Wolbachia primers which used: Taq polymerase: NEB OneTaq Hot Star Quick-Load 2xMM w/ Stand. Buffer (M0488S) Annealing temperatures for PCR reaction: 49 C On April 11th we are running the gel of the results of March 25th using: Taq Polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2x MM w/ Standard Buffer (product #M0488S) DNA Ladder used: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S) |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | We are not confident with our results, there are no clear bands in our samples ( MRH6 and ELMO8) there were distinct smears which indicates that the DNA concentration will need to be reevaluated. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid