Oncopeltus fasciatus

April 11, 2025

Sample information
Picture
Photos by: Madison C.
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by fall 2024 students.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Lygaeidae Oncopeltus Oncopeltus fasciatus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls were all succesful inthe gel electrophoresis and the BLAST DNA results were an exact match.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
>2-B_AB3_Co1_R (1) edited Graph.ab1.scf (622 bp) TCCTGTAGGGTCAAAGAATGAGGTATTAAAGTTTCGATCAGTTAATAGTATTGTGATTGCTCCTGCTAATACAGGTAAGG ATAATAGAAGCAATAGAGCTGTAATTCCTACTGATCATACAAATAAAGGGGTTTTTTCTAAAGACATACCTGTTGGTCGT ATGTTAATAATTGTTGAAATAAAATTAATTGCACCCAGAATTGATGAAATTCCTGCTAAATGTAGAGAAAAAATAGCCAT ATCTACAGATGCTCCTCTGTGAAAAATTCTATTAGATAGAGGAGGATAAACAGTTCATCCTGTTCCAGCTCCTCTTTCTG TAATTCTTCTAGATAAAAGAAGGGTTAATGAAGGTGGTAATAATCAGAATCTTATATTATTTATTCGTGGAAATGCTATA TCAGGTGCTCCAATTATTAAAGGTACTAGTCAGTTTCCGAAGCCACCAATTATAATTGGTATTACTATAAAGAAAATTAT AATAAATGCATGGGCTGTTACAATTACATTATAAATTTGGTCATTTCCAATAAATATACCTGGTTGACCTAATTCAATTC GAATAATTCATCTTATTGATGAACCTACTATTCCAGATCATATTCCAAATATAAAGTATAGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Oncopeltus fasciatus was found to be negative for Wolbachia.
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