Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/23/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Venture High School |
| Observations | This specimen was quite small for it’s species, ( about half an inch long ) leading me to believe that it was quite young. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Dysderidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Followed recommended procedure for wolbachia only primers |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I am very confident that this specimen is wolbachia negative because all my controls showed up as they should. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Dysderidae was found to be negative for Wolbachia. |
Melanoplus femurrubrum
Sehirus cinctus
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus