Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/22/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Venture High School |
| Observations | I found it in the soil under a rock |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Agelenidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | we followed the recommended conditions for wolbachia primers only. we used the standard reagents from MinniPCR |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | cause I didn’t have an arthropod that had Wolbachia. there was no band in my arthropod lane but all my controls were correct. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Agelenidae was found to be negative for Wolbachia. |
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata