coras lamellosus

April 2, 2021

Sample information
Picture
Photos by: Gracie N. Tyson E.
Location
Collection date 03/22/2021
Captive / Cultivated? Wild-caught
Group Venture High School
Observations

I found it in the soil under a rock
Putative identification Arthropoda Chelicerata Arachnida Araneae Agelenidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

we followed the recommended conditions for wolbachia primers only. we used the standard reagents from MinniPCR

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

cause I didn’t have an arthropod that had Wolbachia. there was no band in my arthropod lane but all my controls were correct.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Agelenidae was found to be negative for Wolbachia.
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