Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/27/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | The arthropod is a black beetle, no other coloring. It is small, width at 0.5 inches. Beetle is slightly curved, legs are still intact. There are ridges among the abdomen. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Confidence level of absence of Wolbachia is high – no band of DNA corresponding to Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Flat Backed Millipede
millipede??
tuftlegged orbweaver
Parajulidae Millipede
Banded Longhorn Beetle