Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 03/09/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | Very small (~3 mm), elytron with brown, black, and white “zig-zag” pattern. Flying (wings present). Beetle. Collected late winter, in forest on ground, in 56°F weather on a partly cloudy day. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Dermestidae Anthrenus Anthrenus verbasci |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | CO1 Band Produced WOL Band produced |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | WOL Band produced, high quality FASTA file BLAST produced wolbacia as result |
| Wolbachia 16S sequence | Download FASTA
Download AB1
TGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGT TGCCATCAGGTAATGCTGAGTACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGT CAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGTTGCAAGGT GCGCAAACCTAAGCTAATCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAG TTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCC GTCACGCCATGGGAATTGG
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence |
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| Summary | The Anthrenus verbasci was found to be postive for Wolbachia. |
Baetis21Cb2.1
Diptera21Aa2.1
Baetis21Aa6
Baetis21Aa2.3
Diptera_Cb6