Sample information

Found mating with other water striders as being collected in shallow stream

Methods

Results

There was no clear band for the CO1 test which indicates that this sample did not test positive as an arthropod, indicating error in DNA extraction considering both the positive and negative controls had CO1 bands (meaning that error in gel electrophoresis is less likely).  The 16S test (for Wolbachia presence) had been jostled during the gel loading process (as the loading dye was not visible once the gel had been run), which is why the ladder is not visible. However, the positive and negative controls appear to have the expected result, suggesting that this jostling error had occurred during loading one of the A4-A6 samples. This means that the lack of Wolbachia band in the A5 sample could be due to no Wolbachia presence or this error. The gel to its left (with A1-A3 samples) is added for comparison as it had run for the same duration.