Black-Palped Jumping Spider (Pseudeuophrys erratica)

April 11, 2025

Sample information
Picture
Photos by: Hana A.
Location
Collection date 03/31/2025
Captive / Cultivated? Wild-caught
Group Scientists in Residence
Observations

I found it on the sidewalk at around 2:30 PM. It had just rained and was very sunny and warm.
Putative identification Arthropoda Arachnida Araneae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

My arthropod was in lane 2. The rest are controls except for lane 3 (my partner’s results).

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My confidence level is high because all of the controls worked.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TAGTAGGAACAGCGATAAGAGTATTAATTCGTATAGAATTAGGTCAAACCGGAAATTTTATGGGTGACGATCATTTGTAT AATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGGGGTTTTGGTAATTG ATTGGTTCCTTTAATGTTAGGGGCGCCGGATATAGCTTTTCCACGAATAAATAATTTGAGTTTTTGATTATTACCCCCCT CTTTAATATTATTGTTTATCTCTTCTATAGCGGAAATAGGAGTTGGAGCTGGATGAACGGTGTATCCGCCTTTAGCATCT ATTGTAGGTCATAATGGAAGTTCGGTGGATTTTGCTATTTTTTCCTTGCATTTGGCTGGTGCTTCGTCTATTATAGGGGC TATTAATTTCATTTCTACTGTTATTAATATGCGATCGGTTGGAATATCTTTAGATAGGGTACCTTTGTTTGTATGATCTG TAGTTATTACAGCCGTCTTATTACTTCTTTCATTACCAGTACTGGCTGGTGCTATTACTATATTGTTGACGGACCGTAAT TTTAATACTTCTTTTTTTGACCCTGCAGGAGGGGGGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Araneae was found to be negative for Wolbachia.
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