Captive Blaptica Dubia Roach

April 11, 2025

Sample information
Picture
Photos by: Madison H
Location
Collection date 02/12/2025
Captive / Cultivated? Captive / Cultivated
Group Berkshire Community College
Observations

This Blaptica Dubia roach was purchased from a local Petco store, where it was part of a captive colony.

 
Putative identification Arthropoda Hexapoda Insecta Blattodea Blaberidae Blaptica Blaptica dubia

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

For the DNA extraction we used the insect adaptation of the protocol

On March 4th 2025 we did a single PCR with Arthropod primers which used:

Taq polymerase used: New England Bio Labs One Taq

Annealing temperatures for PCR reaction: 49 C

On March 25th 2025 we did a duplex PCR with Arthropod and Wolbachia primers which used:

Taq polymerase: NEB OneTaq Hot Star Quick-Load 2xMM w/ Stand. Buffer (M0488S)

Annealing temperatures for PCR reaction: 49 C

On April 11th we are running the gel of the results of March 25th using:

Taq Polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2x MM w/ Standard Buffer (product #M0488S)

DNA Ladder used: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

We are not confident with our results, there are no clear bands in our samples ( MRH6 and ELMO8) there were distinct smears which indicates that the DNA concentration will need to be reevaluated.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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