MAC-AGS, Hymenoptera

April 14, 2025

Sample information
Picture
Photos by: Aviva S
Location No map location data available.
Collection date 01/27/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Insects collected on January 27th, 2025. 
  • Collected at 1:15pm EST
  • Insects were collected at the courtyards near the Science center on the Georgia Southern Armstrong campus. 
  • The insects collection site included rotting branches, small amounts of snow, pine straw and leaves. 
  • The insects were collected through a method of sorting through dirt with the soft tweezers. 
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident due to the fact that the possibility of wolbachia in hymenoptera is low, which supports my hypothesis.
Wolbachia 16S sequence
Arthropod COI sequence
GGATCTTCaaTAAGAATACTTATTCGTTTAGAATTacgtatttcAAATTC ATTAATTAATAATGATCAAGTTTATAATTCTATAGTTACTAGACATGCTT TTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGA AATTTTTTAGTTCCTTTAATACTAGGATCCCCAGATATAGCCTATCCTCG AATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTACTTT TATTAAGAAATTTCATTAATGATGGAGTGGGAACAGGATGAACTGTTTAC CCTCCATTAGCTTCTAATATTTTTCATAATGGCCCTTCAGTTGACTTAAC AATTTTTTCTCTTCATATTGCGGGAATATCTTCTATTCTAGGAGCTATCA ATTTTATTTCAACAATTTTAAATATACGACATAAAAATTTTTCTTCTGAT AAAATTCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCT TCTTTCTTTACCAGTTCTTGCTGGAGCTATTACTATACTTTTAACTGACC GAAATCTTAATACTTCCTTTTTTGATCCCTCAGGCGGAGGAGACCCTGTT CTTTATCAACATTTATTTTGATTTTTTGGTCACC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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