Sample information |
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| Picture |
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|---|---|
| Location | No map location data available. |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | found in Roughly 4 inches into soft dirt and mulch, Icy ground, cloudy skies, roughly 45 degrees fahrenheit. Surrounded by trees, bushes and other ground vegetation. |
| Putative identification | |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | “DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All controls and procedures were controlled and successful. Wolbachia was only present in 15% of Dermaptera ordered insects in previous years leading to the conclusion that my results are correct. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
CTTGATCGGGgatgGTAGGAACTAATTTGAGAATTTTAATTCGAACAGAG TTagcgtcagCCTGGATCTTTAATTGGAGATGATCAGATTTATAATGTTA TTGTTACTGCTCACGCTTTTATTATAATTTTTTTTATGGTAATGCCAATT ATAATTGGAGGGTTTGGTAATTGATTAGTTCCTTTAATATTGAGAGCTCC AGATATAGCTTTCCCACGAATGAATAATATAAGATTTTGGTTATTACCTC CATCTCTGATTTTATTATTATCAGGGGGCCTAGTAGATAGAGGGGCTGGG ACAGGTTGGACAGTGTATCCTCCTTTATCATCAGTGATTTCTCATGGAGG GGCTTCAGTGGATATAACTATTTTTTCTTTACATTTGGCAGGGGTTTCTT CTATTTTGGGAGCCATTAATTTTATTACTACTGTGATTAATATACGTCCA GAAGGTTTAAAGCTTGATCGTGTTCCTTTATTTGTTTGATCAGTAGCTAT TACTGCTTTATTATTATTGCTTTCTTTACCGGTTTTAGCAGGAGCTATT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid