MAC-AEJ Dermaptera

April 14, 2025

Sample information
Picture
Photos by: Ava J
Location No map location data available.
Collection date
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

found in

Roughly 4 inches into soft dirt and mulch, Icy ground, cloudy skies, roughly 45 degrees fahrenheit. Surrounded by trees, bushes and other ground vegetation.
Putative identification

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

“DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls and procedures were controlled and successful. Wolbachia was only present in 15% of Dermaptera ordered insects in previous years leading to the conclusion that my results are correct.
Wolbachia 16S sequence
Arthropod COI sequence
CTTGATCGGGgatgGTAGGAACTAATTTGAGAATTTTAATTCGAACAGAG TTagcgtcagCCTGGATCTTTAATTGGAGATGATCAGATTTATAATGTTA TTGTTACTGCTCACGCTTTTATTATAATTTTTTTTATGGTAATGCCAATT ATAATTGGAGGGTTTGGTAATTGATTAGTTCCTTTAATATTGAGAGCTCC AGATATAGCTTTCCCACGAATGAATAATATAAGATTTTGGTTATTACCTC CATCTCTGATTTTATTATTATCAGGGGGCCTAGTAGATAGAGGGGCTGGG ACAGGTTGGACAGTGTATCCTCCTTTATCATCAGTGATTTCTCATGGAGG GGCTTCAGTGGATATAACTATTTTTTCTTTACATTTGGCAGGGGTTTCTT CTATTTTGGGAGCCATTAATTTTATTACTACTGTGATTAATATACGTCCA GAAGGTTTAAAGCTTGATCGTGTTCCTTTATTTGTTTGATCAGTAGCTAT TACTGCTTTATTATTATTGCTTTCTTTACCGGTTTTAGCAGGAGCTATT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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