MAC-MJH Hymenoptera

April 14, 2025

Sample information
Picture
Photos by: Madison H.
Location No map location data available.
Collection date 01/22/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I didn’t really know much about Wolbachia until I did gene sequencing and that has made me more confident in my results. The controls went well and my samples displayed properly
Wolbachia 16S sequence
TGGCgtgCtGATCCACGATTACTAGCGATtccaccttTttgcactcgAGT TgCaaagtACAATCCGAACTGagaTGgctTTTAAGGGATTAGCTTaGcCT CGCGACTTTGCAGCCCATTgTaGCCACCATTgTAGCACgtgtgTAGCCCA CTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTT ATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAG GATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGA GCTGACGACAGCCATGCAACACCTGTGTGAAATCCGGCcgaACCGACCCT ATCCCTTcaaATAG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
CGGCTCCTCAATAAGAATAATTATTCGACTTGAACTTgcttctTGTAATT CTTTAATTAATAATGATCAAATTTATAATACTTTAGTAACCAGACATGCT TTTATTATAATTTTTTTTATAGTAATACCCTTCATAATTGGAGGATTTGG AAATTTTCTAATTCCTTTAATACTTGGATCCCCAGATATAGCCTACCCTC GTATAAATAATATAAGATTCTGACTTCTTCCTCCCTCTCTCTCCCTCCTT CTTATAAGTAGATTTATTAATACAGGAGTTGGTACAGGTTGAACAATTTA CCCACCTTTAGCCTCTAATACCTTTCATAGTGGACCTTCTATTGACCTAT CCATTTTTTCCCTCCATATTGCTGGAATATCATCTATTCTAGGTGCTATC AATTTTATTGCTACAATTATTAATATACACCACCAAAATTTTTCTATAGA TAAAATCCCCTTATTAGTCTGATCTATTTTAATTACTGCCGTACTTTTAC TTCTTTCCCTTCCTGTTCTAGCAGGCGCAATCACTATACTTCTAACAGAT CGAAATTTAAATACATCCTTTTTTGATCCAGCAGGAGGAGGTGACCCAAT TCTTTATCAACACTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be postive for Wolbachia.
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