Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/20/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | |
| Putative identification | |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 0.25 TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Data was sequenced and no Wolbachia was found. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GGgatgGTAGGAACTAATTTGAGAATTTTAATTCGAACAGAGTTAGGTCA GCCTGGATCTTTAATTGGAGATGATCAGATTTATAATGTTATTGTTACTG CTCACGCTTTTATTATAATTTTTTTTATGGTAATGCCAATTATAATTGGA GGGTTTGGTAATTGATTAGTTCCTTTAATATTGAGAGCTCCAGATATAGC TTTCCCACGAATGAATAATATAAGATTTTGGTTATTACCTCCATCTCTGA TTTTATTATTATCAGGGGGCCTAGTAGATAGAGGGGCTGGGACAGGTTGG ACAGTGTATCCTCCTTTATCATCAGTGATTTCTCATGGAGGGGCTTCAGT GGATATAACTATTTTTTCTTTACATTTGGCAGGGGTTTCTTCTATTTTGG GAGCCATTAATTTTATTACTACTGTGATTAATATACGTCCAGAAGGTTTA AAGCTTGATCGTGTTCCTTTATTTGTTTGATCAGTAGCTATTACTGCTTT ATTATTATTGCTTTCTTTACCGGTTTTAGCAGGAGCTATTACGATATTAT TAACTGATCGAAATTTAAATACATCTTTTTTTGATCCAGCCGGAGGCGGA GATCCTATTTTATATCAACATTTATTTTGATTTTTTGGT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid