Sample information
Picture
Location
Collection date	09/03/2024
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	Collected by Fall 2024 students.
Putative identification	Arthropoda Hexapoda Insecta Hymenoptera Formicidae Formica Formica pallidefulva
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level	Unreliable results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Formica pallidefulva was found to be negative for Wolbachia.