Western Honey Bee

April 15, 2025

Sample information
Picture
Photos by: Hailey Bachmann, Dr. Heinz
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 students
Putative identification Arthropoda Insecta Hymenoptera Apidae Apis Apis mellifera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

11-Electrophoresis

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

I had no Wolbachia- therefore my band could not be a high/ medium concentration
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
>62-D_AB2_Co1_R.ab1 (605 bp) ATGTATTAAAATTTCGATCAAATAATAGTATAGTAATTCGCTCCAGCTAATACAGGTAATGATATAATTAATAAAATTGC TGTAATAAAAACTGATCATGGAAATAATGAAATTTGGTCATAATTTATAGAAAAATTTTTTATTATTATAATTGTAACTA TTAAGTTTAATGATCCTATAATTGAGGAAATTCCTGATATATGAAGAGAAAAAATTGCAAAATCTACTGAAGGTGAAGAA TGATATAAATATGCTGATAATGGTGGATATACTGTTCATCCAGTTCCTGGTCTTGGATAAAATAAATTTCTTAATAAAAG TATAAATAATGNGGGAGGAAGTAATCAAAATCTAATATTATTTATTCGGGGGAATGCTATATCAGGTGATCCTAGTATTA AAGGAATAAGCCAATTTCCAAATCCTCCAATTAAAAATGGTATAACTATAAAAAAAATTATTAGGAATGCATGACTAGTA ACAATTGTATTATAAATTTGATCATTGCTAATTCATGATCCTGGGGATCTTAATTCTATTCGAATAATAAGTCTCATTGA TGATCCTAGTATTCCAGATCATAAAGCTAGAATAATATACAAGAT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Apis mellifera was found to be negative for Wolbachia.
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