April 15, 2025

Sample information
Picture
Photos by: Areeba S.
Location
Collection date 01/28/2025
Captive / Cultivated? Captive / Cultivated
Group Benedictine University
Observations

Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Above 99%
Wolbachia 16S sequence Download FASTA   
GGTGGGCGGGGCAGTGTGTACAGACCCGAGACGTATTCACCGTGGCGTGCTGATCCACGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAACCCGGCCGAACCGACCCTATCCCTTCAATAAGGTATGA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA   
GAGGGTGATTGTTTCTCTATTTGTTCTCCTCCTCCTGCTGGATCACAAAATGATCGTATTAAGATTTCGATCAGTTAATAATATAGTAATTGCTCCTGCTAGTACTGGTAAAGATAACAATAAAAGAAATGCTGTAATTACTACTGATCAAACAAATAAAGGTATACGGTCTAAAGGAATTCCTGTAGATCGTATATTAATTACTGTAGTAATAAAATTTACTGCTCCTAAAATTGAAGATACTCCTGCCAGATGTAAAGAAAAAATAGCTAAATCAACCGAAGCACCTCCATGAGCGATTACTGATGATAAAGGTGGGTATACTGTTCATCCAGTACCGGCTCCGTTTTCTACTATTCTTCTTACTAATAGTAAGGTTAAAGCTGGTGGTAAAAGTCAAAATCTTATATTATTTATTCGTGGAAATGCTATATCAGGGGCTCCTAACATTAATGGAACTAATCAGTTTCCGAATCCACCAATTATAATAGGTATCACTATAAAAAAAATTATAATAAATGCATGTGCAGTTACAATTACATTGTAAATTTGGTCATCTCCAATTAGAGTTCCTGGGGCGTCCTAGTTCAGCACGGATTAGAATTCTTAAAGAAGTTCCTACTATTCCTGCTCAAGCTCCGAATATAAAATATAAAGTACCAATATCTTTATATT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
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