MAC-CHS Hemiptera

April 15, 2025

Sample information
Picture
Location No map location data available.
Collection date 01/28/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Georgia Southern Armstrong Campus Hawes Hall
  • Green leaves on the ground
  • Heavy sunlight
  • Dead Grass
  • Sweep Net
  • Green Leafhopper
  • Wings
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My insect had a pure A260/A280 ratio meaning that protein did not contaminate my PCR.
Wolbachia 16S sequence
Arthropod COI sequence
ATTTTTGGATCTGATCaggaaTaGTAGGAATAATATTAAGAATAATTATT CGAATTGAACTAGctcatccAGGACCATTTTTAACAAATGATCAATTATA TAATGTAATTGTAACATCACATGCATTTATTATAATTTTTTTCATAGTAA TACCAATTATAATTGGGGGGTTTGGTAACTGATTATTACCTCTAATAATT GGAGCACCAGATATAGCATTCCCACGAATAAATAATATAAGATTCTGACT TTTACCACCATCACTAACACTTCTATTAGTTAGATCTATAGTAGAAATAG GAGCAGGAACTGGTTGAACAGTATACCCACCATTATCATCTAATGCAGCA CATTCAGGACCTAGAGTTGACCTAGCAATTTTCTCACTACATTTAGCTGG TATTTCATCAATCTTAGGAGCAGTTAATTTTATTACAACACTTATTAATA TACGAAGTATTGGTATAAATTTAGATCGAACACCATTATTTGCATGATCA GTTATAATTACTGCAATTTTACTATTATTATCTCTACCAGTATTAGCTGG GGCAATTACTATACTATTAACAGATCGTAATTTAAATACATCATTCTTTG ACCCAGCTGGGGGTGGGGATCCTATTTTATATCAACATTTATTTTGATTT TTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
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