WRI-MUR-14

April 16, 2025

Sample information
Picture
Photos by: Melanie Urbina-Ramos
Location
Collection date 01/01/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Method of collecting: 

  • Aerial/Sweep net 
  • Large specimen jar
  • Gloved hands 

Description of environment:

A semi-dry landscape with moist, fertile soil, semi green grass and a flower near it. 

 
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

My teacher said I have Wolbachia, but I don’t really think it does because I accidently but my insect into disinfectant on accident.
Wolbachia 16S sequence
Arthropod COI sequence
GTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTaggcattagagG AAGATTAATTGGAAACGACCAAATTTATAATATAATTgtTACAGCTCATG CTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTT GGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCC ACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTT TAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTT TACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTT AGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAG TAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTaTAATACTT GATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAgCaatTCTTTT ATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACtatT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be postive for Wolbachia.
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