WRI-NIW21

Sample information

Picture
Photos by: Nia W.
Location No map location data available.
Collection date 01/29/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

collected tiny insect that from the moist dirt

brown in color with orange stripes

Putative identification Arthropoda Collembola

Methods

Extraction kit pcr
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results

Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Looking at the graph from TopHat, my insect had a 25% chance of having Wolbachia. Yes, the controls did work because I had quality DNA.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Collembola was found to be postive for Wolbachia.
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