Sample information |
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| Picture |
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|---|---|
| Location | No map location data available. |
| Collection date | 01/29/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | found in the dirt in a area that didnt have much sunshine |
| Putative identification | Arthropoda Insecta Hemiptera |
Methods |
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| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | i dont predict that it will be very high in wolbachia |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
CATCCATAAGTATTTTAATTCGAACTGAACTAggcctttcAGGATCACTA ATCGGAGATGATCAAATTTATAATGTAAtcttAACAGCTCATGCTTTTGT TATAATTTTCTTTATAGTTATACCTATTATAATTGGGGGATTTGGAAATT GATTAGTTCCTTTAATATTAGGAGCTCCTGATATGGCATTCCCTCGAATA AATAATATAAGATTTTGATTACTTCCCCCTTCTCTTACTATACTACTTGC TAGAAGTATAGTTGATATAGGTGCAGGAACTGGATGAACTGTTTATCCCC CCCTTTCTGCAGGAATTGCCCATGCTGGGTCATCTGTAGATATGGCTATT TTTTCATTACACCTTGCAGGAGTTTCCTCAATTCTAGGGGCTGTAAACTT TATTACCACAATAATTAATATACGTGCTCCAGGAATATCTCTTGATCAAA CACCTTTATTTGTATGAGCTGTAGGAATTACAGCTCTTCTTCTCCTCCTA TCTCTCCCCGTTCTGGCGGGAGCAATTACTATACTATTAACTGACCGAAA CTTAAATACTTCTTTCTTTGATCCTGCTGGAGGAGGAGATCCAATTCTTT ATCAACATTTATTTTGATTTTTTGGTCACCCTGGAAGTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Hemiptera was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid