WRI-JAL-18 Order Hymenoptera

April 18, 2025

Sample information
Picture
Photos by: Joelin L.
Location
Collection date 01/29/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

 

Afternoon, 1 PM
Dip net, Sweep net, Digging by hand
Dirt (dry and wet), fallen leaves & sticks, straw mulch, flower bed
Georgia Southern Armstrong Campus, surrounding areas of student union
Lady bug, ants, worm, and earwig
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I feel semi confident but also skeptical because I predicted that my insect would not have Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
GATCATCAATAAGAATAATTATTCGAATTGAATTAGgattctgTGGATCC ATTATTAATAATGACCAACTGTATAACTCTATCGTAACAGGACATGCATT TATCATAATTTTCTTTATAGTTATACCATTTATAATCGGAGGATTTGGTA ACTTTTTAATTCCACTAATAATTGGGTCACCAGATATAGCTTACCCCCGT ATAAATAACATAAGATTTTGACTCTTACCCCCATCAATTATACTACTCAC CTTAAGAAGATTTATTAATTCAGGTGCGGGAACTGGTTGAACTGTATACC CTCCCTTATCCTCAAGAATTTTCCATAGGGGAGCCTCAGTAGATTTAGCA ATTTTTTCTCTCCATATTGCAGGTATTTCATCAATCATAGGAGCTATCAA CTTCATCTCTACAATTATTAACATAACCCATAAAAATCTTTCAATAGATA AAACCCCTCTCATAGTATGATCTATTCTAATTACAGCAGTCCTTCTCCTC CTTTCCCTTCCTGTATTAGCCGGAGCAATTACAATACTACTAACAGATCG AAACCTTAATACTTCATTTTTTGACCCAGCAGGGGGAGGAGATCCTATTC TTTACCAACATTTATTTTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be postive for Wolbachia.
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