Harmonia axyridis

April 23, 2025

Sample information
Picture
Photos by: Aiza S
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2024 students.
Putative identification Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

The gel electrophoresis resulted in a barely visible band for the presence of Wolbachia, and the BLAST match was lower than the ideal 99%-100%.
Wolbachia 16S sequence Download FASTA   
ATTANNTTANGNTTGCGCACCTTGNNNNCCATTGTAGACACCATTGTAGCACGTGTGTAGCCCACTCCATAAAGGCCATGATGACTTGACATCATCNNNNCCTTNNNNNNNNTTATCACTGGCAGTTTCCTTAAAGTACTCNNNNTTACCTGATAGCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACTTGTGTAAAATCCNNNNNAACCGACCCTATCCCTTCGAATAGGTATG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA   
TGCTGTAATAAGAACAGATCATACAAATAAAGGAGTTTTATCAAGTATTATACCAAATGGACGTATATTTATAATAGTTG AAATGAAATTTACTGCACCTAAAATTGANGAAATTCCTGCTAAATGTAAACTANNNATCNCTAAATCTACTGAANGCCCA NTANGNGTTAAATTANAAGAAAGAGGAGGGNAAACAGTTCATCCTGTTCCTGCCCCTATTTCTACGATTGTTCTTAAAAT TAAAAGAGTTAAAGCAGGGGGTAAAAGTCAAAATCTTATGTTATTTAATCGTGGAAATGCTATATCAGGAGCTCCAATTA TTAAAGGAACTAATCAATTTCCAAAACCCCNAATTATAATAGGTATTACTATAAAGAAAATTATAATGAAAGCATGAGCT GTAACAATTATATTATAAATTTGNTCGTTTCCNATTAATCTTCCTCTAGTTCCNAATTCTAACCGAATTAAAATACTTAA CGATGTNCCTACTATTCCTGCCCNTATTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Harmonia axyridis was found to be postive for Wolbachia.
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