Polistes Dominula

April 23, 2025

Sample information
Picture
Photos by: Malaika Z
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 students.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Vespidae Polistinae Polistes Polistes dominula

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I chose medium because I had a strong Wolbachia gel band, but my blast match was 95.05%. Our controls did work, I can trust my results due to the gel electrophoresis results indicating thick bands in the gel wells.
Wolbachia 16S sequence Download FASTA    Download AB1
>24-D_WG2_Wo_R.ab1 (659 bp) TGGGCTCGTTCGTATTGCTGAACAGGCGACTATCCACGGGATGCTGACACGATCATCGCGACCCACCCATGACTCAGTTG AGCGTAAATCCGAACCAGACTCTTCAGGGATAGCTTAGGCTTGCGCACCCCGCAACCCATCGTAGACACCATTGTAGCAC GTGTGTAGCCCACTCCATAAAGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGCTTATCACTGGCAGTTTCCTT AAAGTACTCAGCATTACCTGATGGCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACAC GAGCTGACGACAGCCATGCAACACTTGTGTAAAATCCGGCCGAACCGACCCTATCCCTCGACACCCTATGCGATGGACGG CCATTCTGGCCCCTCTCATCAAGGGAACCATTTTCTTCCCAATCCCTCTATTCATAATATGATTTACCTAAAAAAAAATC ATTACAAAAGCATGAGCCGTATTAATTACATTATAAATTTGATCATCTCCAATTAAAGCTCCTGGTTGGCCCATTTCTGC CCGAATCAACATGCTCAATGAAGTCCCCTACTATTCTCTGATCCCCCCCAATACAAAATGTACAAGTTCTCATATCTTTT GTTTTTTTTTGACCAAGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
>58-D_AG2_Co1_R.ab1 (641 bp) TCTCCTCCTGTAGGATCAAAAAAAGATGTATTTAAATTTCGATCAGTTAATAGTATAGTGATTGCTCCTGCTAAGACAGG TAAAGATAATAAAAGTAAAATTGCTGTAATTAAAACAGATCATGTAAATAAAGGTAAAAAATTTAATGAATGGGTTTTAA TATGTATATTTAAAATTGTTACAATAAAATTAATAGCTCCTATAATGGATGAGATTCCAGCAATATGAAGAGAAAAAATT GATAAATCAACAGAAGGTGAATTATGTCCAATATTTGAAGATAAAGGTGGGTATAAAGTTCATCCTGTTCCTACTCCTAT TTTTATTATGTTTCTTATAATAAGCAAAAATAATGAGGGTGGTAAAAGTCAAAATCTTATATTATTTATTCGTGGGAAAG CTATATCAGGGGCTCCAAGTATTATAGGAATTAATCAATTTCCAAAACCTCCAATTATAAATGGTATAACTATAAAAAAA ATTATTACTAAAGCATGGGCTGTAATAATTGAATTATAAAATTGATCATTATTAATAATTGAAGATGGTGTTCCTAATTC TAATCGAATTATTAATCTTAAAGATGTACCAATTATTCCAGCTCAAAGTCCAAAAATAAAATATAATGTTCCAATATCTT T
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Polistes dominula was found to be postive for Wolbachia.
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