NV Northern House Mosquito

April 8, 2021

Sample information
Picture
Photos by: Gio Spatafora
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

It was dead, in a web, outside of house, on garage door.
Putative identification Arthropoda Insecta

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There isn’t any indication of any bands around 500 Bp
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Insecta was found to be negative for Wolbachia.
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