Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/23/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Venture High School |
| Observations | |
| Putative identification | Arthropoda Myriapoda Chilopoda Lithobiomorpha |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | we used recommended conditions for Wolbachia Primers only. we used standard reagents for MiniPCR. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I had to do the expirement twice to get it right so I know for sure that this anthropod has no Wolbachia |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lithobiomorpha was found to be negative for Wolbachia. |
Water Boatman