Stone Centipede

Sample information

Picture
Photos by: Nicholas B, Tyson E
Location
Collection date 03/23/2021
Captive / Cultivated? Wild-caught
Group Venture High School
Observations
Putative identification Chilopoda Lithobiomorpha

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

we used recommended conditions for Wolbachia Primers only. we used standard reagents for MiniPCR.

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

I had to do the expirement twice to get it right so I know for sure that this anthropod has no Wolbachia

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lithobiomorpha was found to be negative for Wolbachia.
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