Pill Bug GRP10

May 21, 2025

Sample information
Picture
Photos by: Giselle C., Ofelia L.
Location
Collection date 04/30/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Location: CAMS bushes

Season: Spring

Temperature: 62°F

Habitat: Terrestrial

  • pointy tail
  • attatched on leaf
  • very still
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit tweezers
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence Download FASTA   
Arthropod COI sequence
NNNNNNNNNNGNNTTTGGGGTTTGAGCAGGGGCTGTTGGAACTGCCCTTAGAATAATTATTCGCACTGATTTTTGGGTCN CCCTGAATTTNANTGGAGATGACCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTA ATACCTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCATTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAAT AAATAATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGGTTGAAAGAGGAGTAGGAA CAGGATGGACAGTATATCCTCCGCTGGCGTCTAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTC CACCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATTAG AATAGACCGTGTTCCTTTATTTGTTTGATCAGTATTAGTAACGGCTGTACTTTNACTTTTATCCCTACCTGTATTANCAG GAGCTATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTT TATCAACACTTATTTTGATTTTTTGGTCACCCTTGANNTTTAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
Report Inappropriate Post