Long-Legged Fly GRP10

May 21, 2025

Sample information
Picture
Photos by: Giselle C., Ofelia L.
Location
Collection date 04/30/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations
  • found on bush
  • located in leaf at the top of the bush
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

There were no bands in the lane of the long-legged fly, which means there was either an error in extracting or gel electrophoresis, because the gel cannot confirm if the fly is an arthropod (despite being one), which means it cannot confirm if it has Wolbachia present as well.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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