Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/30/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | |
| Putative identification | Arthropoda Malacostraca Isopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system Bio-Rad |
| Buffer | 1X TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Based on our gel, there was only one visible clear band which indiicates that the roly poly is an arthopod but does not have Wolbachia present. The controls were successful which is the reason for the confidence level in our result. |
| Wolbachia 16S sequence | Download FASTA
|
| Arthropod COI sequence | Download FASTA
NNNNNNNNNNNNNNNNGGTANGANNNNNNGTNNNTGAGGTGCTTGCNCANT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Isopoda was found to be negative for Wolbachia. |
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Wolbachia data
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