Murgantia Histrionica GRP28

May 23, 2025

Sample information
Picture
Photos by: Diego Foster, Eric Shelton, Cameron Acevedo, Osvaldo Madera, Randy Atilano, Dean Cornett
Location
Collection date 05/09/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Bug was found on the leaves of a bush.
Putative identification Arthropoda Insecta Hemiptera Pentatomidae Murgantia Murgantia histrionica

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Body without legs
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain 100X Fast Blast
Gel images
Protocol notes

Gel electrophoresis was run successfully and bands of harlequin bug DNA were present.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

During Gel Electrophoresis the bands for wolbachia presence as given by the DNA positive did not appear and the barcodes between the harlequin bug and the wolbachia are visually different meaning that it can be seen no wolbachia is present.
Wolbachia 16S sequence
Arthropod COI sequence
NNNNNNNNNNNNNNTGNNNNNNNANANNNGGTATAGTTGGTACTTCTTTAAGAATCCTAATTCGCCTAGAACTAGGAACA ACTAATAGATTAATTGGAAACGACCAAATTTATAATGTAATTGTTACTGCTCATGCATTTATTATAATTTTTTTCATAGT AATACCTATTATAATTGGAGGATTTGGAAATTGACTAGTACCATTAATAATTGGCGCTCCAGATATAGCTTTCCCACGTT TAAATAATATAAGATTTTGACTTTTGCCTCCCGCATTAACATTATTAATAATAAGAATAATTGTAGAAATAGGGGCAGGC ACAGGTTGAACAGTCTACCCTCCACTATCTTCAAATTTGGCACATAATGGACCCTCTGTTGATTTAGTAATTTTTAGTTT ACATTTAGCTGGAATTTCGTCTATTTTAGGGGCTGTTAATTTTATTTCAACTATTATAAATATACGCCCTTCGGGAATAA GATTAGATAAAACTCCCTTATTTGTTTGATCAGTTATGATCACAGCTATTTTATTACTTTTATCTTTACCTGTATTAGCA GGAGCCATCACTATACTTTTAACAGATCGAAATATCAATACTTCATTTTTTGACCCTACGGGAGGGGGAGACCCTATTCT TTACCAACATTTATTCTGATTTTTTGGTCACCCTTGAAANTNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Murgantia histrionica was found to be negative for Wolbachia.
Report Inappropriate Post