PillbugWolbachiaGRP5 Sample 2

May 23, 2025

Sample information
Picture
Photos by: Genesis O., Emily M., Adrian B., Crystal K., Jeremiah C.
Location
Collection date 05/01/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

At home, found in the backyard under mulch.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TAE
DNA stain UView
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

PCR ran successfully and thus the bands were clear showing there to be no Wolbachia band present. Along with that, submitted samples lab results were run through BLAST and confirmed to be Armadillidium Vulgare.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
NNNNNNNNNTTTGGGGTTTGAGCAGGGGCTGTTGGAACTGCCCTTAGAATAATTATTCGCACTGAATTAGGACNACCTGG AAGTTTAAATGGAGATGACCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATAC CTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCATTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAATAAAT AATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGGTTGAAAGAGGAGTAGGAACAGG ATGGACAGTATATCCTCCGCTGGCGTCTAGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTCCACC TTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATTAGAATA GACCGTGTTCCTTTATTTGTTTGATCAGTATTAGTAACGGCTGTACTTTTACTTTTATCCCTACCTGTATTAGCAGGAGC TATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTTTATC AACACTTATTTTGATTTTTTGGTCACCCTGAAGTTNAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Armadillidium vulgare was found to be negative for Wolbachia.
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