Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/05/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | shiny green fly |
| Putative identification | Arthropoda Insecta Diptera Dolichopodidae Condylostylus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system Bio-Rad |
| Buffer | 1X TAE |
| DNA stain | 100X Fast Blast |
| Gel images |
|
| Protocol notes | Condylostylus of interest is in the second lane from the left. PCR with Wolbachia 16S primers was performed, so the band indicates that the organism was positive for Wolbachia. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The band was visible in person, but does not come out well on the image. Controls worked well. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
NNNNNNNNNNNNCNNNNNNNNNNNNNNNNGGATAATTGGTACATCCCTTA GAATTATTGTACGAGCTGAATTAGGACATCCAGGAGCTCTAATTGGAGAT GACCAAATTTATAATGTTATTGTAACAGCTCACGCATTTATCATAATTTT CTTTATAGTAATACCAATCATAATTGGAGGGTTTGGAAATTGATTAGTTC CCCTTATATTAGGAGCTCCTGATATAGCATTCCCACGAATAAATAATATA AGATTCTGAATACTCCCCCCATCATTAACCCTACTACTAGCTAGTAGTAT AGTAGAAAATGGAGCAGGAACAGGATGAACAGTATATCCCCCTTTATCAG CAGGAATTGCTCACGGAGGTGCATCTGTTGACTTAGCAATTTTTTCTCTT CATTTAGCTGGTATTTCATCTATTTTAGGAGCAGTAAATTTTATTACAAC AGTAATTAATATACGATCAACAGGAATCACTCTAGATCGAATACCATTAT TTGTATGATCAGTAGTAATTACAGCTGTTCTTCTTCTCCTTTCATTACCA GTTCTAGCCGGAGCTATTACCATATTACTAACTGACCGAAATTTAAATAC TTCATTTTTTGACCCAGCAGGAGGAGGTGACCCAATTCTATATCAACATT TATTTTGATTTTTTGGTCACNCNNNANNNTNNN
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Condylostylus was found to be postive for Wolbachia. |
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Wolbachia data
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