Minettia flaveola GRP7

May 21, 2025

Sample information
Picture
Photos by: Jeyuk B.
Location
Collection date 05/05/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Striped abdomen, red eyes, fly
Putative identification Arthropoda Insecta Diptera Lauxaniidae Minettia Minettia flaveola

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain 100X Fast Blast
Gel images
Protocol notes

Minettia flaveola of interest is in the third lane from the left. PCR with Wolbachia 16S primers was performed, so the lack of band indicates that the organism was negative for Wolbachia.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked well, and no band was visible.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
NNNNNNNNNNNNNNNNGNNNNNNNNNNCCGGATAGTAGGAACATCTTTAA GAATTTTAATTCGTGCTGAATTAGGACACCCAGGTGCCTTAATTGGAGAC GACCAAATTTATAATGTTATTGTTACAGCCCATGCATTTGTTATAATTTT TTTTATAGTTATACCAATTATAATTGGAGGATTTGGAAATTGACTAGTCC CTTTAATATTAGGAGCTCCTGATATGGCATTTCCACGAATAAATAATATA AGATTTTGATTATTACCTCCTTCCCTTACATTACTTTTAGTAAGAAGTAT AGTTGAAAACGGAGCTGGAACAGGATGAACTGTATACCCTCCGTTATCAT CAGGAATTGCTCACGGAGGAGCATCTGTTGATTTAGCTATTTTTAGTCTT CATTTAGCTGGGGCTTCATCAATTATAGGAGCAGTAAATTTTATTACTAC AGTCATTAACATACGATCAACTGGAATCACTTTTGATCGAATACCTTTAT TTGTATGATCTGTTGTAATTACTGCTATTCTTTTACTTCTTTCTCTACCT GTTTTAGCAGGAGCAATCACTATGTTATTAACAGATCGAAATTTAAATAC TTCATTTTTTGACCCAGCGGGTGGAGGAGATCCAATTTTATACCAACATT TATTTTGATTTTTTGGTCACCNNNGNANTTNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Minettia flaveola was found to be negative for Wolbachia.
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