Aphid Group 4

May 25, 2025

Sample information
Picture
Photos by: Kimi P.
Location
Collection date 04/28/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Aphid was collected at the California Academy of Math and Science between 55-65 degrees fahrenheit
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TAE
DNA stain UView
Gel images
Protocol notes
lane 2: mm ruler
lane 3: uni. ruler
lane 5: long leg spider
lane 6: roly poly
lane 7: dragon fly
lane 8: black aphids
lane 9: (+) arthro control
lane 10: (-) arthro control
lane 11: (+) DNA control
lane 12: water
lane 14: mm ruler

lane 15: uni ruler

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

There were no bands in lane 8 which could lead us to presume no wolbachia present in the aphid because there were bands present for the control however we cannot be exactly sure, and would have to reexamine the data for more certainty
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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