Sample information

Methods

During the process of DNA extraction, there was some difficulty separating the tissue from the exoskeleton, possibly leading to a lower quality extraction.

Gel 1
Lane
1 – PCR MW Ladder (failed)
2 – centipede (lamyctes emarginatus)
3 – pillbug (armadillidium vulgare)
4 – woodlouse spider (dysdera crocata)
5 – ground beetle (anisodactylus binotatus)
6 – Arthropod Positive Control
7 – Arthropod Negative Control
8 – Drosophila Positive Control
9 – Drosophila Negative Control

Gel 2
Lane
3 – PCR MW Ruler
6 – MiniOne Ladder

Our first gel’s ladder did not run successfully, so we ran another gel with the same ladder and and another MiniOne ladder and compared the relative mobilities of the two gels to determine the base pairs of the first gel.

Results

Although our controls worked,  we are unsure of why our ladder did not run, so the results of our gel are somewhat questionable. However, all of the samples ran correctly, with aligned bands that were only in expected locations. As stated before, there were some complications with the DNA extraction, but we were also able to visualize a band for Wolbachia when we ran our gel. Our Sanger Sequencing results also had a less than ideal contiguous read length value.