Sample information |
|
| Picture |
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|---|---|
| Location | |
| Collection date | 04/01/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | Dead in house, unknown for how long but body seemed to be in decent condition. |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Tetramorium Tetramorium caespitum |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Lanes 4 and 8 are the Wolbachia and CO1 tests respectively. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Controls worked and bands showed up well, but the specimen is probably older than freshly dead. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TNANTNGCTATTTGAGCAGGAATAATCGGATCTTCAATAAGTATAATTATCCGACTTGAATTAAGTTCCTNTNNTTCATTAATTAATGACGACCAAGTATATAATTCCTTAGTAACAAGACATGCCCTTGTTATAATTTTCTTTATAGTAATGCCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCATTAATACTAGGAACTCCCGATATAGCCTACCCCCGTATAAATAACATAAGATTTTGACTTCTACCACCCTCTATTAGATTATTATTGCTTAGAAGATTTATTAATCCGGGGGTAGGAACAGGATGAACTATCTATCCCCCATTAGCATCTAATATCTTTCATAGAGGCCCATCAATTGATCTATCAATTTTTTCTCTTCATATTGCAGGAATATCTTCTATTATAGGTGCAATTAATTTTATCGCTACTATTATAAACATACATCATAAAAAATTAACCTTAGATAAAATCCCCCTGTTAGTTTGATCTATTTTAATTACAGCAATTCTTCTCCTCCTATCTCTTCCCGTATTAGCAGGAGCTATTACTATACTCTTAACAGATCGTAATTTAAATACCTCATTTTTTGATCCATCAGGTGGGGGGGACCCAATTCTATATCAACATTTATTCTGATTTTTTGGTCAC
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Tetramorium caespitum was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid