NV 66 Minges

April 14, 2021

Sample information
Picture
Location
Collection date 10/22/2026
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations
  • It went from my patio chair to under my patio table
  • It flew in short distances
  • It is a brown/black bug with transparent wings
  • It was moving around a lot and was more by man made structures
Putative identification Arthropoda Hexapoda Insecta Diptera Chironomidae

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The bands on the gel for my sample matched up with the wolbachia control sample.
Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence
Summary The Chironomidae was found to be postive for Wolbachia.
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