|Captive / Cultivated?||Wild-caught|
|Group||Neuqua Valley High School|
The fly was flying at a rather fast pace and was very anxious it seemed. I had to trap it with a banana since it was so fast. As it is incredibly small, it is hard to distinguish many features without a magnifying glass.
|Putative identification||Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster|
|Extraction kit||Puregene (Qiagen)|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR|
|Gel electrophoresis system||Standard electrophoresis system|
|Explanation of confidence level||
The controls were constant through the experiment, and the gel electrophoresis was done in a method repeated by others prior and therefore reliable results. Therefore, I am confident that my arthropod is infected with Wolbachia.
|Wolbachia 16S sequence|| Download AB1
|Arthropod COI sequence||
|Summary||The Drosophila melanogaster was found to be postive for Wolbachia.|