NV 44 Fruit Fly

April 14, 2021

Sample information
Picture
Photos by: Nick N.
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

The fly was flying at a rather fast pace and was very anxious it seemed. I had to trap it with a banana since it was so fast. As it is incredibly small, it is hard to distinguish many features without a magnifying glass.
Putative identification Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The controls were constant through the experiment, and the gel electrophoresis was done in a method repeated by others prior and therefore reliable results. Therefore, I am confident that my arthropod is infected with Wolbachia.
Wolbachia 16S sequence Download AB1
Arthropod COI sequence
Summary The Drosophila melanogaster was found to be postive for Wolbachia.
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