NV 44 Fruit Fly

Sample information
Picture	Photos by: Nick N.
Location
Collection date	10/04/2020
Captive / Cultivated?	Wild-caught
Group	Neuqua Valley High School
Observations	The fly was flying at a rather fast pace and was very anxious it seemed. I had to trap it with a banana since it was so fast. As it is incredibly small, it is hard to distinguish many features without a magnifying glass.
Putative identification	Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster
Methods
Extraction kit	Puregene (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR
Gel electrophoresis system	Standard electrophoresis system
Buffer	LB
DNA stain	GelRed
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	The controls were constant through the experiment, and the gel electrophoresis was done in a method repeated by others prior and therefore reliable results. Therefore, I am confident that my arthropod is infected with Wolbachia.
Wolbachia 16S sequence	Download AB1
Arthropod COI sequence
Summary	The Drosophila melanogaster was found to be postive for Wolbachia.

The Wolbachia Project Database