NV 68 Black Soldier Fly

April 14, 2021

Sample information
Picture
Photos by: Delaney M.
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

The bug didn’t move a lot especially when I put it in a cup and tried to transfer it to the Ziploc bag. It was damp outside today because during the night it rained and so the plant that the bug was resting at was pretty moist. The plant was also a few feet in the air and hangs so it isn’t for an insect to get there.
Putative identification Arthropoda Hexapoda Insecta Diptera Stratiomyidae Hermetia Hermetia illucens

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Its very obvious my insect does not have any dna that would indicate it to have wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Hermetia illucens was found to be negative for Wolbachia.
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