Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/04/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Neuqua Valley High School |
| Observations | The bug didn’t move a lot especially when I put it in a cup and tried to transfer it to the Ziploc bag. It was damp outside today because during the night it rained and so the plant that the bug was resting at was pretty moist. The plant was also a few feet in the air and hangs so it isn’t for an insect to get there. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Stratiomyidae Hermetia Hermetia illucens |
Methods |
|
| Extraction kit | Puregene (Qiagen) |
| DNA extraction location | |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | LB |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Its very obvious my insect does not have any dna that would indicate it to have wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Hermetia illucens was found to be negative for Wolbachia. |
Melanoplus femurrubrum
Sehirus cinctus
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus