Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/29/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | North Penn-Mansfield JSHS |
| Observations | Location – Under fallen leaves on riverside Notes – Capable of quick (~3 inch) evasive jumps |
| Putative identification | Arthropoda Insecta Hemiptera Cicadellidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | All controls returned as expected, and the test lane has two lines in parallel with the Wolbachia DNA lane. One of the lines in the test lane is fairly faint, though it is still defined and visible. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Cicadellidae was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid