Northern Crab Spider Mesaphesa Asperata

September 11, 2025

Sample information
Picture
Photos by: Ethan Levandoski
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Found in small leafy fern right to lion statue outside the main entrance of campus. Small spider of a greenish color with a yellow and black abdomen. Red eyes, superior leg pairs about double in length to inferior two leg pairs. Small brown hairs growing in density on distal side of legs and back of abdomen.
Putative identification Arthropoda Arachnida Araneae Thomisidae Misumena

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Green
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Our confidence level is due to the positive identification, checking DNA through a data base and the 4 controls for each step of the process.
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
NNNNNNNNNNNGNGNNNTGATCTGCTATAGTTGGGACTGCTATAAGAGTGTTGATTCGAATGGAGTTAGGAAATTCTGGA AGATTATTAGGAAATGATCATTTATATAATGTAATTGTTACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTTATACC AATTTTGATTGGTGGGTTTGGGAATTGATTAGTACCTTTAATATTGGGGGCACCTGATATATCTTTCCCACGTATAAATA ATCTATCTTTTTGATTACTTCCGCCTTCATTGTTTTTGTTATTTATATCATCTATGGTTGAAATAGGAGTAGGAGCAGGG TGAACTGTTTATCCTCCTTTAGCATCTAGTGTAGGGCATATAGGAAGATCTATGGATTTTGCTATTTTTTCTCTTCATTT AGCTGGAGCTTCATCTATTATAGGGGCTGTAAATTTTATTTCTACTATTATTAATATGCGAAGTCAAGGTATAAGAATGG AAAAGGTACCTTTATTTGTTTGATCTGTATTAATTACTGCTATTTTACTATTATTATCTCTTCCTGTTTTAGCTGGGGCT ATTACTATATTATTGACTGATCGTAATTTTAATACTTCATTTTTTGACCCTGCTGGAGGAGGGGATCCTATTTTATTTCA ACATTTATTTTGATTTTNNNNNNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Misumena was found to be negative for Wolbachia.
Report Inappropriate Post