white-jawed jumping spider (Hentzia mitrara)

November 20, 2025

Sample information
Picture
Photos by: Evan Malone
Location
Collection date 10/16/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

white-jawed jumping spider (Hentzia mitrara)

Praying Mantis

European Harvestman Spider

Banded Tussock Moth Caterpillar
Putative identification Arthropoda Arachnida

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Line 1 Hentz Spider: has Wolbachia

Line 2 Praying Mantis: has Wolbachia

Line 3

Line 4

Line 5

Line 6

Line 7

Line 8 Empty

Line 9 Harvestmen Spider: no Wolbachia

Line 10 Caterpillar: no Wolbachia

Line 11

Line 12

Line 13

Line 14

Line 15

Line 16 Empty

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Wolbachia 16S sequence
GNNNNNNNGGNGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCA ACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGAT GATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGA GGCTAAGCCAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTA ATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GGNNGCCTGATCTGCTATAGTGGGTATAGCAATAAGAGTATTGATTCGAATAGAATTAGGTCAAACTGGA TCATTTTTAGGTAATGATCATATATATAATGTTATTGTTACAGCTCATGCTTTTGTTATAATTTTTTTTATAGTAATGCC TATTTTAATTGGAGGTTTTGGAAATTGGTTGGTTCCTTTGATATTAGGGGCTCCTGATATAGCTTTTCCTCGGATAAATA ATTTAAGTTTTTGATTATTACCCCCTTCATTATTATTGTTATTTATTTCTTCAATAGCAGAAATAGGAGTTGGGGCTGGA TGAACTGTTTATCCCCCTTTAGCTTCTATTGTTGGTCATAATGGAAGATCTGTAGATTTTGCTATTTTTTCTTTACATCT AGCAGGTGCTTCTTCAATTATAGGAGCTATTAATTTTATTTCAACTATTATTAATATACGTTCTATTGGAATATCTTTAG ATAAGATTCCTCTTTTTGTTTGATCAGTTATAATTACGGCTGCTCTATTACTTTTATCATTACCAGTGTTAGCTGGAGCT ATTACTATATTATTAACTGATCGAAATTTTAATACTTCCTTTTTTGATCCAGCAGGAGGAGGAGATCCTATTTTATTTCA ACATTTATTTTGATTTTTTGGNCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arachnida was found to be postive for Wolbachia.
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