Dissosteira carolina

November 19, 2025

Sample information
Picture
Photos by: Jennifer P.
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

I found the Carolina Grasshopper in front of my university’s parking garage, on the wall by the entrance. I used a sweep net to catch it. It was in the small flower garden that’s in the middle of the pathway by the entrance, and I chased it all the way to the parking garage.
Putative identification Arthropoda Insecta Orthoptera Acrididae Dissosteira Dissosteira carolina

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

When I put my Wolbachia 16S into NCBI Blast, the results showed as 100%.
Wolbachia 16S sequence Download FASTA    Download AB1
CGGTCTCGCGGTTCGATTATACAGACCCGAGACGTATTCACCTGTGGCATGCTGATCCACGATTACTAGCGATTCCAACTTCATGTACTCGAGTTGCAGAGTACAATCCGAACTGAGATGTCTTTTAGGGATTAGCTTAGGCTTGCGCACCTTGCAGCCCATTGTAGACACCATTGTAGCACGTGTGTAGCCCACTTCATAAAGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGCTTATCACTGGCAGTTTCCTTAAAGTACTCAGCATTACCTGATAGCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACTTGTGTAAAATCCGGCCGAACCGACCCTATCCCTTCAAAATAGGTATG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
AGCATTAATGAATGATATAGGATTGTGATCTCCACCTCCAGCTGGATCAAAGAATGATGTATTAATATTACGGTCTGTTAATAGTATTGTAATTGCTCCAGCTAAAACTGGTAATGATAATAGTAATAATAATGCTGTAATTGCTACTGATCAAACAAATAAGGGTGTTTGATCTATAGTTATTCTATCTGATCGTATATTAATTGCTGTTGTAATGAAGTTTACTGCTCCTAAAATTGATGAAATACCTGCTAAGTGTAGTGAAAAAATAGCTAAATCTACTGATGCCCCTCCATGTGCAATTGCTCCTGCTAATGGTGGGTAGACTGTTCATCCTGTACCTACCCCTCTATCAATTATTCTAGATGAGAGTAGTAAAATTAATGATGGTGGTAATAATCAAAATCTTATATTATTTATTCGTGGAAATGCTATATCTGGTGCCCCAATTATTAATGGTACTAGCCAATTTCCAAAACCCCCAATCATGATTGGTATAACTATGAAGAAGATTATAACAAATGCATGTGCTGTGATAATAACATTATAAATTTGGTCATCTCCAATCATAGATCCTGGTTGCCCTAATTCAGCCCGAATAATTATTCTTATAGATGTTCCAACTATACCTGCTCATGCTCCAAATATAAAGTATAAAGTTCCAATATCTTTATGATTTGTTGACC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Dissosteira carolina was found to be postive for Wolbachia.
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