Sample information

Location Description: Specimen collected while it sat on the railing of a terrace.

Season: Autumn

Temperature: 79 Fahrenheit

Habitat: Urban

Notes: Appears to be a spotted lanternfly, has two large brownish wings, a grey body/head, and six legs.

Methods

DNA Extraction: No errors made. Changed pipette tips between each sample.

PCR: Pipette tip still held a bit of DNA solution after depositing 2 uL into the B-3 0.2 mL PCR Tube.

Gel electrophoresis, Arthropod CO1: In Lane 1 is a DNA Ladder, in Lane 2 is Sample #1, in Lane 3 is Sample #2, in Lane 4 is Sample #3, in Lane 5 is Sample #4, in Lane 6 is a Positive Wolbachia Control, in Lane 7 is a Negative Wolbachia Control, and in Lane 8 is Water. No errors were made during this procedure and controls worked as intended.

Gel electrophoresis, Wolbachia: In Lane 1 is a DNA Ladder, in Lane 2 is Sample #1, in Lane 3 is Sample #2, in Lane 4 is Sample #3, in Lane 5 is Sample #4, in Lane 6 is a Positive Wolbachia Control, in Lane 7 is a Negative Wolbachia Control, and in Lane 8 is Water. No errors were made during this procedure and controls worked as intended.

Results

No mistakes were made during our gel electrophoresis and the controls for our Wolbachia and Arthropod CO1 gels were consistent with expected results, giving our sample results confidence.