Lycorma delicatula – Spotted Lanternfly

November 21, 2025

Sample information
Picture
Photos by: Miriam T., Piper M.
Location
Collection date 09/09/2025
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Spotted lantern fly. Gray wings, red underwings with white spots and brown tips. Black body. Long leg-like appendage coming out of the butt, 6 black legs.

Caught in the alley near the school.

Early fall

Temperature: 69ยบ and sunny

Habitat: urban
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

In DNA extraction the filter was clogged. We had to centrifuge it at a higher speed (10000 cpm), and multiple times. Some liquid had to be removed from the tube. We split it into two tubes then recombined the extracted DNA.

PCR: all went well

Gel electrophoresis, Arthropod CO1: DNA ladder in lane 1, C-5 in lane 2, C-6 in lane 3, C-6 in lane 4 (accidentally split C-6 into two lanes, but both showed the same results and there was no contamination.) W+ in lane 5, W- in lane 6, H2O control in lane 7. Controls worked.

Gel electrophoresis, Wolbachia 16S: DNA ladder in lane 1, C-5 in lane 2, C-6 in lane 3, W+ in lane 4, W- in lane 5, H2O control in lane 6. No mistakes were made. Controls worked.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All the controls worked and there was no contamination. The lines were strong.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Hemiptera was found to be negative for Wolbachia.
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