Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 06/04/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | Collected live off of a crabapple tree. |
| Putative identification | Arthropoda Insecta Hymenoptera Philanthidae Cerceris Cerceris nigrescens |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Results are third row. Top: CO1. Bottom: Wol. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Test worked fine. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TTAGNGAGCATCNATTCNAGTATAATAATTCGTTTAGAACTTAGATCTCCAGGTAGATTTATTAATAACAGACAATTATATAATAGAATTGTAACTACACATGCATTTATTATAATTTTTTTTATAGTTATACCATTCATAATTGGTGGATTTGGAAACTGATTAATTCCTATAATATTAGGAGCTCCAGACATAGCATTTCCACGAATAAATAATATAAGATTTTGACTTTTACCACCTTCATTAATTTTTTTATTATTAAGANCAATTACAAATCAAGGATTAGGAACAGGANGAACTTTATACCCACCACTTTCTAGAAATACTGGACACAGAAGANTNNCTGNTGATATATCTATTTTTTCATTACATATAGCAGGAANTTCNTCTANTATAGGNGCTANTAATTTTANTGNAACAATTATTAATANACATAATAAAAANANTGNCATAGAANNNTACAATTACNTAATTGNCNNTTTAACAACTGCNANTCTTTTACTCTTATCTTACCAGACTTGCTGGNNANCACTATACTTTATTGANCGAATTAATACNTCATTTTTGANCCATCAGGAGGGGGGACCNNNNTTNNCACTTAT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Cerceris nigrescens was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid