Wolbachia(+) Agrilus cuprescens

October 21, 2025

Sample information
Picture
Photos by: Elijah M.
Location
Collection date 06/04/2025
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Collected live off of a raspberry bush.
Putative identification Arthropoda Insecta Coleoptera Buprestidae Agrilus Agrilus cuprescens

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Test results are the second row. Top: CO1. Bottom: Wol.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Clear, solid lines. BLAST search matched.
Wolbachia 16S sequence
TTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCTATCAGGTAATGCTGAGTACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGCTGCAAGGTGCGCAAGCCTAAGCTAATCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GGGGCCTGATCAGGNATAGTGGGTACCGCTCTTAGATTATTAATCCGAGCAGAACTCGGAAACCCCGGAGCTTTAATTGGAAATGATCAGATTTATAATGTTATCGTAACTGCTCACGCTTTTATTATAATCTTCTTTATGGTAATACCTATCATAATAGGGGGTTTCGGAAATTGACTTGTTCCTCTAATGCTAGGCGCTCCTGACATAGCCTTCCCTCGGATAAACAATATAAGATTCTGACTACTCCCCCCTTCTTTAACCTTACTTTTAATAAGTAGAATAGTAGAAAGAGGAGCAGGAACAGGTTGAACTGTTTACCCTCCTTTAGCAGCAAATATTGCTCACAGAGGAGGTTCTGTAGATTTGGCAATCTTTAGTTTACATTTAGCAGGGATCTCCTCTATTTTAGGTGCCATTAATTTTATTACAACAGTAATTAATATACGAGCTGTAGGAATAACAATAGACCGGGTACCTTTATTAGTTTGGTCAATTGCTATCACTGCACTCCTTCTCTTGTTATCTTTACCAGTACTAGCAGGAGCCATTACTATACTACTCACTGACCGAAATTTAAATACATCCTTCTTTGACCCGGCAGGGGGAGGAGACCCTATTCTTTATCAGCATTTATTCTGATTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Agrilus cuprescens was found to be postive for Wolbachia.
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