Sample information
Picture
Location
Collection date	09/17/2025
Captive / Cultivated?	Wild-caught
Group	St. Paul Academy and Summit School
Observations	Black, smaller than a grain of rice
Putative identification	Arthropoda Insecta Hymenoptera Formicidae Tetramorium Tetramorium caespitum
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	SeeGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence	Download AB1 CO1F BLAST at The Wolbachia Project   BLAST at NCBI
Summary	The Tetramorium caespitum was found to be negative for Wolbachia.