Sample information
Picture
Location
Collection date	09/17/2025
Captive / Cultivated?	Wild-caught
Group	St. Paul Academy and Summit School
Observations	Collected far from the building in the trees.
Putative identification	Arthropoda Insecta Hymenoptera Formicidae Formica Formica glacialis
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	SeeGreen
Gel images
Protocol notes	Lanes 6 and 7
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	The gel is bright
Wolbachia 16S sequence	Download AB1
Arthropod COI sequence	Download AB1
Summary	The Formica glacialis was found to be postive for Wolbachia.